Reagent Development Services
Developing reagents for the target and the application simultaneously
There is a significant unmet need for a new generation of affinity reagents, that can be designed for any target, and optimised specifically to meet the requirement of a chosen cell and gene therapy application. To tackle this, MIP Discovery is taking a multifaced approach to development; we are developing a catalogue of licensable reagents, as well as offering a custom development service for more unique targets and applications.
Expanding catalogue of licensable affinity reagents
Polyethylenimine (PEI) is a commonly used transfection reagent, however it is challenging to measure the presence of this toxic compound in the final product. Current methods are time consuming, involving several steps; separation from DNA, followed by HPLC coupled to various detection methods. MIP Discovery is utilizing synthetic affinity reagent technology to develop a reagent with the potential to reduce the complexity of PEI testing, that can easily be integrated into testing methods such as SPR and BLI.
Custom development to meet your exact requirement
For clients looking for a more unique affinity reagent, or those looking for a package of reagents on an accelerated timeline, we offer a custom reagent development service. This service consists of three phases:
Epitope discovery
- Desktop assessment
- Proprietary in-silico modelling and/or in-vitro epitope discovery
- Proprietary monomer library
- Template generation
Chemical Manufacture
- Monomer selection & reagent synthesis
- Reagent screening & analytics
- Refinement of synthesis
- Generation of lead candidates
Analytics
- Screening using FDA
- Side analysis using Des and Nanosight
- Affinity analysis using SPR
Prototype Delivery
- Proofing of principle Reagent delivery
- Technical support
Epitope discovery
We utilize both in-silico and in-vitro techniques to assess the surface structure of the target and identify regions/epitopes that are most suited to reagent development. We then screen a library of over 400 monomers, the building blocks of non-biological affinity reagents, to select those that will combine to create the best reagent for the end application.
Chemical Manufacture
The carefully chosen building blocks are taken to the lab, where a controlled polymerization is carried out in the presence of the target, creating the imprinted polymer particles. Multiple purification steps are then used to release the polymer-based affinity reagents from solution.
Analytics
The prototype affinity reagents are then characterized using a range of techniques including, but not limited to FIDA, DLS and SPR. The prototype synthetic reagent is then delivered.
