Viral Vector Purification
We are designing synthetic affinity ligands for a new wave of capture chromatography solutions
To support the industry on the journey to improved viral vector purification, Tozaro is developing synthetic affinity ligands for a new wave of capture chromatography resins and membranes. Purification of viral vectors remains a complex process, with multiple steps and associated equipment for debris removal, washing, concentration, purification, and polishing. Smart polymers offer exciting possibilities to streamline processes, enable rapid R&D and produce greater batch sizes.
Innovative synthetic affinity ligands for capture chromatography
High quality biological affinity ligands for capture chromatography of viral vectors are rare, if available at all. Smart polymers have the potential to transform the bioprocessing landscape by enabling efficient capture chromatography with delicate recovery.
Tozaro is designing synthetic affinity ligands to drive fresh possibilities in Lentivirus capture chromatography.
Transforming downstream processing for Lentivirus
Tozaro is developing a suite of smart polymers that bind the VSV-G membrane protein of lentivirus with gentle release conditions. With the development pipeline of lentivirus based therapies growing, scalable purification that does not impact viral infectivity is urgently required. There are extremely limited commercially available capture chromatography solutions to date, and releasing the viral particles without impacting infectivity remains a challenge. By utilizing our in-silico design process and chemical manufacture, we aim to achieve extremely mild release conditions to ensure the viral infectivity remains intact.
Why choose smart polymers?
Designed in silico.
- Our pioneering computational chemistry means we can screen the full surface of any viral particle, engineered or not, to identify optimally exposed epitopes and produce synthetic affinity reagents that are truly novel.
- We have access to over 600 monomer building blocks (versus the traditional 20 amino acids) allowing us to build-in the exact functionality needed for the application.
Synthesized chemically.
- The chemical manufacturing process of smart polymers means we can deliver a new wave of synthetic affinity reagents that go beyond the capabilities of biologics, providing safe, reproducible and scalable affinity solutions to our clients.
- Smart polymers are free of Animal Derived Components (ADCs), reducing the risk of leached ligands on product quality and safety.
- Robust purification formats allow for sterilization, increased cleanability and re-use potential.
Deployed with confidence.
- Smart polymers offer new solutions to solving high value bioprocessing challenges for our clients. We are using our technology to go beyond what is currently possible.
- Large batch sizes, rapid scale up and manufacturing consistency means clients can depend on the smart polymer supply chain.
