There are very few dedicated affinity solutions that have been developed specifically for lentivirus purification – instead, existing techniques for other targets have adapted. This means that there is nearly always a compromise: Lentivirus Smart Polymers Magnetic Beads are designed specifically for the application, therefore they overcome a number of these challenges.  

Smart Polymer™ ligands combine selective VSV-G binding with controlled affinity, allowing efficient capture and gentle elution of lentiviral vectors. This helps manufacturers achieve strong functional recovery while reducing host cell proteins (HCPs) and residual DNA in a simplified workflow. 

VSV-G (Vesicular Stomatitis Virus Glycoprotein) is commonly used to pseudotype lentiviral vectors because it improves vector stability and broadens cell targeting capabilities. By specifically targeting VSV-G, Smart Polymer™ ligands can selectively capture lentiviral particles expressing VSV-G while minimizing the co-purification of process-related impurities lacking VSV-G. 

Tozaro’s first generation of Lentivirus Smart Polymers have been designed to specifically bind to the VSV-G protein on lentiviruses. However, the design process means that they can be designed to bind other surface regions. Please get in touch if you would like to discuss a custom development to a different pseudotype.  

Tozaro is working with industry partners to integrate Smart Polymer™ ligands onto GMP-compatible purification formats, including membranes and monoliths. These collaborations are focused on developing scalable purification solutions suitable for clinical and commercial lentiviral vector manufacturing.

Smart Polymer™ ligands can be incorporated into a range of purification and analytical formats, including magnetic beads, membranes, monoliths and biosensor platforms. This flexibility allows the technology to support research, process development and manufacturing applications. Smart Polymer™ Magnetic breads are available to purchase directly from Tozaro.  

Yes. Tozaro has developed high-affinity Smart Polymer™ ligands for lentiviral vector analytics. These ligands have been demonstrated on commercial Biolayer Interferometry (BLI) platforms and enable rapid lentivirus detection and quantification across a broad range of vector titers.